FastQCFastQC Report
Mon 20 Mar 2023
SRR6211782_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211782_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148887127
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA51740883.475174855110207No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14502860.9740842134726665No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13411490.9007823759001005No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12647570.8494737090332867No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA7001290.4702414601633088No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6427470.4317008548361606No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2457760.16507538626895527No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2137830.14358729616698157No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2046570.13745782064825524Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2043910.1372791618176634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA16196750.0142.072721
GACAACT8327300.0141.269593
CGACAAC16382550.0141.062122
GGCTAAC2957250.0139.9262
ATGAATC2691350.0139.21862
AGTTAGC2110550.0138.88212
ACAGTGC3909900.0138.356952
GATGTAC3171850.0138.11552
TGGAACA3529150.0137.287371
GACAACG3406800.0137.227863
GACAGTG3928050.0137.08371
GATAGAC1361500.0136.93071
ATCAGCA2268500.0136.235892
TGGTGAC2761950.0136.121355
AGGCTAA3014700.0135.921
ATAGCGA1432550.0135.616331
GCGAGTA2047200.0135.012391
CGAGTAA2036700.0134.829132
TATCAGC2296950.0133.788681
GTACGCA1511900.0133.721561
Produced by FastQC (version 0.11.5)