FastQCFastQC Report
Mon 20 Mar 2023
SRR6211781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119722735
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3862499932.26204195886437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32589892.7221137238470203No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7278670.6079605515193084No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4127690.3447707739052236No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4123030.3443815412335844No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3969370.3315468862284177No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3944380.32945956338200927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3552970.2967665247540494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3179480.2655702778590883No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3162400.2641436482385739No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3084000.25759518440670437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA3053410.2550401141437339TruSeq Adapter, Index 2 (95% over 22bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2844890.2376232049827462No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2728850.2279308103009842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2676710.22357574774749342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2576730.21522478583537205No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2381440.19891292994601234No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2291790.19142479496479928No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2238940.18701042872099438No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2183770.18240228140461376No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2101740.1755506170152227No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2075310.17334301626169832No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1997610.16685302085689907No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1948900.1627844535960526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1922730.1605985696868686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1824560.15239879042188603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1806660.150903669215375No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1710310.14285590786077515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1629570.13611199242984218No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1570590.1311856098175505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1558970.13021503392818415No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1548250.12931963173076524No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1494030.1247908344225514No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1481130.12371334483797085No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1442910.12052096871993444No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1380810.11533398397555819No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1349030.11267951738656821No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1321570.11038588451892618No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1239900.10356428960631413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1220870.10197478365324682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT245466100.045.5875781
AGCAGTG248864450.044.8948862
AGTGGTA250365950.044.5970275
CAGTGGT251059800.044.5099644
GCAGTGG252152200.044.2868543
TGGTATC252594050.044.1246537
GTGGTAT253283800.044.0815126
GTATCAA253967650.044.0194859
GGTATCA254230400.043.9019248
TGACTGG399200.022.3705522
GTGACTG404050.022.335421
ACCTAGC656700.020.58762735-39
CGTTCCA312100.020.36294790-94
ATGGGGC2805850.020.27161825-29
AAACCGC747000.019.86870285-89
TACTACC695400.019.59706960-64
ATGGGGG3808550.019.59510225-29
GAAACCG779500.019.52627685-89
AACGAAC135300.019.5191825
TAGCCCC682000.019.51368335-39
Produced by FastQC (version 0.11.5)