FastQCFastQC Report
Mon 20 Mar 2023
SRR6211777_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211777_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107474293
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA9364000.0142.171332
GATCGCA3776100.0142.123052
ACGTGAT5289400.0141.921262
CAGATCT6449750.0141.643481
ATCCTGT9407450.0141.514711
AACATCG5160200.0141.40372
AACGTGA5309800.0141.3761
AGATCGC3800500.0141.21061
CCACTGT9623800.0141.168462
ACGTATC2331450.0141.165791
ATGCCTA4122400.0140.400151
ACGCTTA2789050.0140.350132
CGCATAC2690000.0140.253881
AGATCTG6518550.0140.14852
CCTGTAT4464800.0140.092733
CACTGTA2945900.0140.025423
CACTGTG3461200.0140.007113
ACTAGTA3515000.0139.916382
GAGCTGA2661650.0139.627231
CAAGCTA2965700.0139.498372
Produced by FastQC (version 0.11.5)