FastQCFastQC Report
Mon 20 Mar 2023
SRR6211777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107474293
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18536711.724757565979057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13151121.2236526180265266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA8568590.7972687943153067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5564390.5177414844682905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4000330.3722127299781353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2659920.24749360295861633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1801510.16762240994690703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1599930.1488662967989936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1588640.14781581303354097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1544160.1436771489159738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1536100.14292720213567722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1418940.13202599062456732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1346390.12527553914683578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1268520.11803008557590604No Hit
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC1086160.10106230705793058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT49980400.0138.494381
AGCAGTG50267650.0137.41742
GGTATCA50139050.0137.207188
GTGGTAT50359500.0137.170586
TGGTATC50298000.0137.030557
GTATCAA50354700.0136.88929
CAGTGGT50551950.0136.651474
AGTGGTA50623900.0136.468815
GCAGTGG52688100.0131.073523
GTATCGA131850.064.568199
GGTATCG143900.059.1114048
GGTATAA370900.047.613748
TGGTATA402750.044.116417
TTGTATC493600.030.7770967
TGTATCA508600.029.826958
GCAGTTG515500.029.6801263
GTTGTAT530850.028.6852386
CAACGCA50123350.027.78662310-14
ATCAACG50222100.027.69372610-14
CGCAGAG50255200.027.67769615-19
Produced by FastQC (version 0.11.5)