FastQCFastQC Report
Mon 20 Mar 2023
SRR6211776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28180058
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18064966.410547487162731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA9124653.237981270301147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5630201.9979376905469817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1914610.6794201772047452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1619370.5746510528828578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1512090.5365815783629686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1388520.4927314202121231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1346610.4778592009995153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1029580.36535765824186733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA797370.2829554147830356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC702020.2491194304852034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA698920.24801936177704106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA664540.23581924494264703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA657790.23342393404584194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG648620.230169859834923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA642410.22796617380986228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA540910.19194779513938545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA490600.17409474458853136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA486650.1726930441378084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA465470.16517709083494436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA443300.15730982526721554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAAAAAAAAAAAAAAAAA437870.15538293072356346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGAGAAAAAAAAAAAAAAAAAA427240.1516107596371874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA393640.13968743428420197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAAAAAAAAAAAAAAA377750.13404869500268593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA376930.13375770908633333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGAAAAAAAAAAAAAAAA370890.13161434940978475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA361160.12816155310964938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC359400.12753699797211207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT343390.12185567538576393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT329740.11701182446111359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGGAAAAAAAAAAAAAAAAAAA289790.10283513256076336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT18021050.0141.239211
AGCAGTG18157800.0140.152452
GTGGTAT18162200.0140.066746
GGTATCA18141900.0139.987058
TGGTATC18182400.0139.920627
CAGTGGT18201750.0139.799424
GTATCAA18192400.0139.628429
GCAGTGG18241250.0139.451263
AGTGGTA18275250.0139.216835
GGTATCG33400.090.4936458
GTATCGA35550.085.0204549
CAACGCA18120550.028.31584210-14
ATCAACG18140450.028.2426510-14
TACATGG17829150.028.24202520-24
CGCAGAG18150350.028.23382615-19
GTACATG18018100.028.19002220-24
TCAACGC18202800.028.17753410-14
GCAGAGT18102250.028.1757515-19
AGTACAT18045450.028.1695120-24
CATGGGG7409350.028.1644125-29
Produced by FastQC (version 0.11.5)