FastQCFastQC Report
Mon 20 Mar 2023
SRR6211774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98907311
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC2361300.0141.179982
GTACGCA3838000.0141.097141
GTCGTAG3601800.0140.8091
GATGTAC3867200.0140.752272
TACGCAA3850450.0140.640932
TCGTAGA3610650.0140.463872
ATAGCGA2374300.0140.406971
TCACGCA2799400.0139.794822
GATAGAC2292950.0139.6611
TATCAGC2051500.0139.533191
CTCGGTA3083600.0139.526052
GCGAGTA2827150.0139.507541
CGAGTAA2845700.0138.598142
GTGCGAA3715600.0138.544542
GCCATGC2055200.0138.269552
GGTGCGA3723100.0138.265461
ATCAGCA2072650.0138.109342
ATGAATC3043400.0138.094852
GCTCGGT3116750.0138.042021
TTCACGC2839800.0137.806051
Produced by FastQC (version 0.11.5)