FastQCFastQC Report
Mon 20 Mar 2023
SRR6211774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98907311
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA44514104.50058742371431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA26885462.718247996854348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11592101.1720164953225753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11156921.1280177256057442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6869160.6945047773060983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3586640.36262637854950885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA3214890.32504068379737877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA2572430.26008491930389255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2378640.24049182774769803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2133790.21573632711539395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1529050.15459423419164636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA1170970.11839064151688444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA1147640.11603186745214415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT44475100.0140.854526
GGTATCA44426200.0140.846858
AAGCAGT44604350.0140.703691
TGGTATC44575300.0140.581677
GTATCAA44613900.0140.272679
CAGTGGT44896550.0139.817754
AGCAGTG44928350.0139.726322
GCAGTGG45257700.0138.655533
AGTGGTA45681350.0137.157535
GGTATCG112100.065.8325658
GTATCGA118450.062.4249049
CAACGCA44384550.028.51940710-14
ATCAACG44395500.028.42826810-14
CGCAGAG44489150.028.3966815-19
TCAACGC44715250.028.31276910-14
GTACATG44251700.028.30518220-24
TACATGG44093650.028.29818520-24
CAGAGTA44506800.028.26790615-19
AGAGTAC44460350.028.23574315-19
AGTACAT44365950.028.22349520-24
Produced by FastQC (version 0.11.5)