FastQCFastQC Report
Mon 20 Mar 2023
SRR6211773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36530526
Sequences flagged as poor quality0
Sequence length151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28747707.869500701960876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6657421.8224265371924837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5271511.443042457149399No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1188250.32527590760669584No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA778590.2131340786059308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA647920.17736399415655826No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC606240.16595435828107155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG554330.15174432473269067No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT553440.15150069287258552No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT537110.14703045885515034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAAGAAAAAAAAAAAA519640.14224815706185015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA514320.14079184077447995No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT503930.13794764411549945No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG481410.13178293682384973No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG457350.12519666429111914No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA428590.11732379654210291No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC425440.11646150400352845No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG400540.10964528679384469No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAAAAAAAAAAAAAAA398050.10896366507287632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC391210.10709125841768607No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA380640.10419778789935846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG370520.10142750202939864No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA368550.1008882270132108No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG367180.10051319819484668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT28719650.054.7699851
AGCAGTG29176450.053.6217422
CAGTGGT29353850.053.1762354
GCAGTGG29383450.053.099643
AGTGGTA29421950.053.0304835
GTGGTAT29441900.052.9648976
GGTATCA29473600.052.9381878
TGGTATC29482200.052.7815977
GTATCAA29734500.052.7501839
CGAACTT43400.027.5821821
CGAAGTA45350.026.5397622
CGCATAC42750.026.4742031
CCGAAGT48550.026.0012631
ACGTATC48650.025.798691
CATGCGA79000.023.585844145
CGTGGGA161700.022.86681145
ATGGGGT835450.021.64334525-29
CATGGGG3349900.019.07523325-29
AAACCGC188250.018.9403385-89
GGTATAA242550.018.8308988
Produced by FastQC (version 0.11.5)