FastQCFastQC Report
Mon 20 Mar 2023
SRR6211771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52531022
Sequences flagged as poor quality0
Sequence length151
%GC31

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8585271.6343238096528945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6464081.230526221248846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2840910.540806154504285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2547580.4849667687790274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2005020.38168303673970017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAAAAAAAAAAAAAAAA1474680.28072554918120574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTTTTTAAAAAAAAAAAA1104630.21028145997235692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1083330.2062267130458646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1042110.19837992110642738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG880260.16756955537624985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA789250.150244554541505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA770500.14667523506395896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA732270.13939763060387442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA730820.1391216032309442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAATGAAATAAAGCA693300.1319791570017427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC665920.12676699874599814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCTTACCAAAAAAAAAAA659740.12559055104619893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATATGTGAGATGTTTAAATA648650.12347941755254638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA640320.12189368788598857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAATGACCCAAAAAAAAA590930.11249162447286862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTTTGACCAACCGAACAT584740.11131327313601476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA564060.10737655170691329No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTGGAAATGACCCAAAAA558760.10636762406792694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT31082350.0138.519821
AGCAGTG31592200.0136.00372
GTGGTAT31575800.0135.663996
GGTATCA31410600.0135.65578
CAGTGGT31573550.0135.638984
TGGTATC31451600.0135.378577
GTATCAA31566650.0135.338999
AGTGGTA31678700.0135.256275
GCAGTGG31934150.0134.170823
TGGTATA345400.077.788577
GGTATAA374000.071.859418
GTCTGTC315150.056.3476871
TCTGTCA322050.054.3927542
GCAGTTG262100.046.1420443
GTCGTAG47600.045.2432861
TGTATCA291650.041.712168
GTATAAA693700.039.0034149
TTGTATC326400.037.3379337
GTTGTAT325350.037.258596
CAGTTGT327100.037.0592544
Produced by FastQC (version 0.11.5)