FastQCFastQC Report
Mon 20 Mar 2023
SRR6211769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75326938
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3814750.0142.06291
ACACAGA9710750.0141.560361
CGTATCA3839450.0141.148972
ACAGCAG3689250.0140.465091
CAGCAGA3689850.0140.442252
AGAGTCA3850900.0140.246921
GAGTCAA3851750.0140.215972
GATCGCA2173800.0140.148792
GATAGAC1982850.0140.032331
GCATACA2880500.0139.433322
CACAGAA9879050.0139.148732
AACATCG2105850.0138.851882
CGCATAC2895950.0138.689441
AGATCGC2202150.0138.344531
ATGCCTA2425150.0138.047911
AGGTACA2857450.0137.846912
CTCAATG2766600.0137.335241
AAGGTAC2871900.0137.153321
GAGCTGA1523300.0136.92661
ACATCGT868650.0136.894153
Produced by FastQC (version 0.11.5)