FastQCFastQC Report
Mon 20 Mar 2023
SRR6211768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73046048
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2678180.3666426963988524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC967940.13251093337725814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA941320.12886665682447324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA821730.11249479232606806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA775530.1061700148377637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT54182000.0139.629941
AGCAGTG55273850.0136.830872
AGTGGTA54243300.0136.611485
CAGTGGT54562400.0136.307054
TGGTATC54274650.0136.28737
GTGGTAT54389900.0136.228186
GGTATCA53997450.0135.693628
GTATCAA54112500.0135.351149
GCAGTGG56763100.0133.213493
GTATCGA406150.0129.938239
GGTATCG408300.0129.465528
GTGGGAT1213600.095.486236
CAGTGGG1295050.090.418354
GGATCAA1383250.080.705969
AGTGGGA1576500.073.588085
AGTGGTC374400.069.729095
GTGGTCT477300.054.8471226
GGGATCA2044700.054.6824658
GTATCCA423150.052.4448179
TGGGATC2374150.048.3734747
Produced by FastQC (version 0.11.5)