FastQCFastQC Report
Mon 20 Mar 2023
SRR6211767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79747595
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA6579700.0141.488891
AACGTGA5859500.0141.105671
ACGTATC2799500.0141.047011
GTGGTCA8466850.0141.047012
GATAGAC3004250.0140.960481
GATCGCA3069000.0140.949462
ACGTGAT5879800.0140.61852
AGTGGTC8497800.0140.533331
AGATCGC3088450.0140.061811
TGCCTAA6650950.0139.973142
CGTATCA2826300.0139.709562
GAGTCAA3500250.0139.597092
AGAGTCA3503350.0139.473571
CGAAGTA3151450.0139.066732
GTCACTA1944100.0138.738042
CGTGATG2637750.0138.603333
ATAGACA3058350.0138.4672
GAGCTGA2157400.0138.37361
AGTCACT1960200.0137.598511
TGGTCAG3296000.0137.327883
Produced by FastQC (version 0.11.5)