FastQCFastQC Report
Mon 20 Mar 2023
SRR6211767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79747595
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3871460.48546416979722085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1451420.18200172682323523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA878760.11019266474430985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC822770.10317176336164119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT61442250.0139.373641
AGCAGTG62700900.0136.541532
AGTGGTA61505600.0136.312235
CAGTGGT61846300.0136.021644
TGGTATC61561900.0135.956157
GTGGTAT61681200.0135.904796
GGTATCA61305450.0135.286428
GTATCAA61447350.0134.912759
GCAGTGG64064950.0133.586583
GTATCGA450050.0129.877129
GGTATCG453550.0129.27158
GTGGGAT1368650.099.8674246
CAGTGGG1443150.095.515014
GGATCAA1612250.081.9975749
AGTGGGA1942200.070.571995
AGTGGTC409050.066.372375
GGGATCA2312800.057.2817468
GTGGTCT490100.055.396086
GTATCCA429450.053.5346769
GGTATCC479000.048.1620458
Produced by FastQC (version 0.11.5)