FastQCFastQC Report
Mon 20 Mar 2023
SRR6211766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57745465
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC4698750.0142.81261
CATACCA9823700.0142.684431
CGTATCA4711850.0142.415562
ATACCAA9906450.0141.492582
AACGTGA4489200.0141.35721
ACGTGAT4506200.0140.823912
TACCAAG3482650.0140.319263
ACACAGA4767300.0140.133831
CAAGCTA3782600.0139.836212
GTATCAG1656750.0139.229783
CGTGATG2009850.0139.042793
ATGCCTA2172450.0138.843551
CACAGAA4819950.0138.603122
GAGCTGA1456850.0138.496551
ACAAGCT3826700.0138.22471
TACCAAT3600250.0138.193593
GATAGAC919800.0137.531451
GTCACTA1087600.0137.403612
CTGTAGC1976100.0137.110931
AACATCG1183100.0136.986482
Produced by FastQC (version 0.11.5)