FastQCFastQC Report
Mon 20 Mar 2023
SRR6211766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57745465
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2853950.49422928709639796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1108160.1919042473724993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1017200.17615236105553916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA993270.1720083126874119No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG942940.16329247673388725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA935880.1620698699023378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT734140.127133793103926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA695970.12052375022003892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC685480.11870715734993216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT41768050.0138.264761
AGCAGTG42567950.0135.63092
AGTGGTA41775700.0135.341355
GTGGTAT41824600.0135.169176
CAGTGGT41991200.0135.158264
TGGTATC41792900.0135.041527
GGTATCA41524900.0134.627088
GTATCAA41627200.0134.24029
GCAGTGG43459500.0132.823533
GTATCGA316000.0125.859679
GGTATCG317900.0125.650718
GTGGGAT942800.095.382886
CAGTGGG1019750.089.226334
GGATCAA1090550.079.537129
AGTGGGA1334800.067.484345
AGTGGTC327650.063.6461945
GGGATCA1565100.055.503638
GTATCCA326550.054.3189629
GTGGTCT395300.052.5902146
TGGGATC1947000.045.8289577
Produced by FastQC (version 0.11.5)