FastQCFastQC Report
Mon 20 Mar 2023
SRR6211765_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211765_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75023935
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13061501.7409777293073203No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11081251.4770286309295828No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT2256290.3007426896496431No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1910740.2546840551618627No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1138650.15177156463467292No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1022490.1362885057948507No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT966870.12887487173260107No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC792210.1055943013386328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGAC6672000.0141.612435
CTAAGTG6964100.0141.266053
TAAGTGA6171200.0140.672594
CCTAAGT7214600.0139.649112
GTCACTA1830500.0138.697422
AGTGACT3530700.0138.30366
AGCTGAA1800600.0138.03682
GTGACTG3253550.0137.970847
AGTGACC2348200.0137.931176
CTCAATG1755950.0137.370931
TGACTGG3137150.0136.967458
GCCTAAG7613900.0136.956741
CGAACTT791650.0136.44861
CAATGGA3439800.0135.545851
AGAGTCA912600.0135.44181
TGACCGG1920900.0135.35158
GACTAGT1426350.0134.967471
GAGCTGA1867400.0134.864691
TCAATGA1785650.0134.550322
CAAGCTA815950.0134.477452
Produced by FastQC (version 0.11.5)