FastQCFastQC Report
Mon 20 Mar 2023
SRR6211761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83968590
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA4819800.0143.28471
ATAGCGA3203150.0143.273471
CGACAAC4823100.0143.186652
TAGCGAC3206500.0143.123782
ATCAGCA3009300.0143.085682
ATCATTC5680400.0142.851431
AGGCTAA4206900.0142.821491
ACCTTAC3293300.0142.74892
CCTAATC3605800.0142.699361
TATCAGC3017650.0142.689761
ATCCGTC3313100.0142.65642
ACACGAC2238700.0142.591811
CTAATCC3616100.0142.29292
TACGCAA1171200.0141.9442
TCATTCC5721350.0141.828982
CTTCACA3598250.0141.558092
CACGACC2256150.0141.488942
GTACGCA1175800.0141.388691
AGCCATG2221050.0141.365021
GGCTAAC4253850.0141.245152
Produced by FastQC (version 0.11.5)