FastQCFastQC Report
Mon 20 Mar 2023
SRR6211760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83500752
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC5145450.0143.1222
ATCATTC6178050.0143.107331
CAGATTC5650400.0143.015382
ATAGCGA2423350.0142.986391
TAGCGAC2424050.0142.945082
AGGCTAA3736700.0142.672821
CCGACAA2893850.0142.670441
CGACAAC2897050.0142.512852
GCGAGTA5627650.0142.465131
TCACGCA2419850.0142.157782
CCTAATC2114750.0142.066131
ATCAGCA1014300.0141.817352
TCATTCC6234800.0141.804762
GGCTAAC3763750.0141.647432
CACCTTA5201200.0141.58791
CTAATCC2122900.0141.520722
AGATTCG1880250.0141.319933
ATCCGTC1418900.0141.215852
CCTTACG1679600.0141.167363
CGACACA1096500.0141.054891
Produced by FastQC (version 0.11.5)