FastQCFastQC Report
Mon 20 Mar 2023
SRR6211760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83500752
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9503071.138081966016306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2668890.3196246663742621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2333330.27943820194577407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1107370.13261796732082126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1090790.13063235646069393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT1033870.12381565138479232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG985260.11799414692696421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT889660.10654514824010208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT29795000.0135.718831
GTGGTAT29782800.0135.504876
TGGTATC29677500.0135.285757
AGTGGTA29887800.0135.062555
GGTATCA29775950.0134.924538
CAGTGGT29909300.0134.893954
AGCAGTG29953450.0134.624012
GTATCAA30045500.0134.195249
GCAGTGG30506900.0132.120453
GGTATAA456350.051.164158
TGGTATA478150.048.876637
GTATAAA778850.030.1356549
GTTGTAT494750.029.526586
CAACGCA29753400.027.39620410-14
ATCAACG29781250.027.33001710-14
CGCAGAG29846050.027.27193615-19
TCAACGC29926500.027.21185510-14
TTGTATC539400.027.1758887
AACGCAG30098750.027.08918410-14
AGAGTAC30072900.027.04361315-19
Produced by FastQC (version 0.11.5)