FastQCFastQC Report
Mon 20 Mar 2023
SRR6211759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68209834
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10458291.5332525219164146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4068800.5965122272545041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2097720.30753923253940185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA784540.11501860567495296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24106950.0131.750956
AAGCAGT24265900.0131.677351
TGGTATC23925900.0131.509527
AGTGGTA24185500.0131.34215
GGTATCA23980400.0131.3378
CAGTGGT24228400.0131.032884
AGCAGTG24297550.0130.721922
GTATCAA24262450.0130.645729
GCAGTGG24894850.0127.574833
GGTATAA417950.073.388658
TGGTATA444200.069.0355457
GCAGTTG433200.046.578923
GTATAAA688500.044.9056439
GTTGTAT457700.044.320926
TTGTATC461250.043.8542447
TGTATCA487350.041.62388
CAGTTGT541950.037.4840474
AGCAGTT587450.034.7038042
AGTTGTA696800.029.2262765
CATGGGG9219400.026.74895725-29
Produced by FastQC (version 0.11.5)