FastQCFastQC Report
Mon 20 Mar 2023
SRR6211758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72718603
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5409680.74391968173536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2071560.2848734594090043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1565870.21533279455327273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT33995300.0139.941391
TGGTATC33901650.0139.397557
AGTGGTA34075200.0139.325265
GTGGTAT34112300.0139.120766
GGTATCA34033000.0138.939948
AGCAGTG34141500.0138.93882
CAGTGGT34187700.0138.793914
GTATCAA34330850.0138.246229
GCAGTGG35224750.0134.597023
TGGTATA436600.056.2821357
GGTATAA443550.055.2541588
GTTGTAT389950.045.862816
GCAGTTG405700.044.3127063
CAGTTGT413850.043.5273634
TTGTATC421250.042.5747227
GGTGCGA137350.034.0998541
AGTTGTA533150.033.8280035
TGCGAAT49050.033.4933473
TGTATCA548150.032.7972268
GTATAAA751200.032.740129
Produced by FastQC (version 0.11.5)