FastQCFastQC Report
Mon 20 Mar 2023
SRR6211757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50931689
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC4666850.0143.533941
CAGATCT4412450.0142.142211
CGTATCA4714950.0142.069662
AGATCTG4437000.0141.355742
ACGCTTA1308200.0139.736052
ATGCCTA1242650.0138.974731
CGCATAC1405900.0138.647021
AGTCACT1111200.0138.55681
GTATCAG1512750.0138.491183
GTGGTCA3027400.0137.994782
AGTGGTC3029600.0137.894581
GTCACTA1118400.0137.664812
AACATCG1545300.0137.375472
CGAACTT1181350.0137.134611
GAGCTGA1582800.0137.010831
ACTTCGA726000.0136.391952
GATCGCA1023700.0136.30032
GATAGAC1318650.0136.061281
TGCCTAA1269650.0136.019352
GCTGATC831950.0135.944522
Produced by FastQC (version 0.11.5)