FastQCFastQC Report
Mon 20 Mar 2023
SRR6211757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50931689
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3358230.6593596375725925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2175360.4271132653778672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC834240.16379586390704615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA768940.15097476936215487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT728720.14307791756130453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG697550.1369579555863541No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA664900.13054740831390846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT601750.11814844781605417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC591300.11609668000603711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT541190.10625801158881654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT27594300.0142.286471
AGCAGTG28165000.0139.275832
AGTGGTA28224600.0138.940355
TGGTATC28134450.0138.923817
GTGGTAT28242050.0138.838356
CAGTGGT28282100.0138.655824
GGTATCA28238800.0138.416878
GTATCAA28389100.0137.852849
GCAGTGG28901150.0135.691993
CGCTGTT216900.050.04354145
TGGTATA233300.041.4525647
GGTATAA245300.039.3951648
TGCGGCT221250.036.049164145
CACGCAG172300.035.854206145
AGAGAGT1345850.031.942623145
GTTGTAT233200.030.1856826
TTGTATC235900.029.5328797
CAACGCA28209600.028.45981210-14
GCAGTTG246800.028.4328353
AACGCAG28339350.028.36859910-14
Produced by FastQC (version 0.11.5)