FastQCFastQC Report
Mon 20 Mar 2023
SRR6211756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51394356
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1893510.3684276148921878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1381240.26875324597899425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1219340.23725173246649886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1081500.21043166685462505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT26880600.0139.985051
AGCAGTG27360500.0136.9112
GTGGTAT27376900.0136.74236
TGGTATC27228550.0136.697367
AGTGGTA27417100.0136.566675
CAGTGGT27436950.0136.431664
GGTATCA27318250.0136.297078
GTATCAA27543250.0135.74859
GCAGTGG27915050.0134.094643
GGTATAA363350.050.5385448
TGGTATA362950.050.5343177
GTTGTAT397000.041.0505076
GCAGTTG401000.040.9183923
TTGTATC415050.039.1080677
CAGTTGT441400.037.1347854
TGTATCA445200.036.4921468
CACGCAG213750.033.310165145
CGGTCTG84200.031.947231145
AGCAGTT525850.031.4855772
GTATAAA616600.029.9694679
Produced by FastQC (version 0.11.5)