FastQCFastQC Report
Mon 20 Mar 2023
SRR6211755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55797875
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4621790.8283093218155709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4515500.8092602092821635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1989120.35648669416174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA673210.12065154811003106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC609560.10924430365851746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT577980.10358458991493852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT25993300.0133.213491
AGCAGTG26467250.0130.459562
GTGGTAT26467250.0130.383936
AGTGGTA26499350.0130.248145
GGTATCA26412000.0130.206248
TGGTATC26425200.0130.121447
CAGTGGT26586250.0129.793784
GTATCAA26594500.0129.65439
GCAGTGG27010350.0127.745563
CGGTCTG103200.041.240036145
GGTATAA276350.037.408758
TGGTATA287550.035.9516877
CGCTGTT195950.034.004066145
GCTCGAC57450.027.386106145
AGAGAGT935450.027.19715145
CAACGCA26388100.026.8007810-14
ATCAACG26447000.026.64443810-14
AACGCAG26594350.026.6249310-14
CGCAGAG26452050.026.61912515-19
TCAACGC26587200.026.59277210-14
Produced by FastQC (version 0.11.5)