FastQCFastQC Report
Mon 20 Mar 2023
SRR6211754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66562330
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC11786000.0143.630041
ATAGACA11807950.0143.363042
ACGTATC4542300.0142.978351
TAGACAT4267650.0142.459033
ATGCCTA3420450.0142.316221
GAGTCAA2554100.0142.183382
CGAAGTA2655950.0141.995382
ACTAGTA2673850.0141.922592
AGAGTCA2559900.0141.861241
GACTAGT2678850.0141.65771
CGTATCA4586500.0141.600462
TGCCTAA3441400.0141.449842
TAGACAA3470200.0141.327823
TAGACAG4194050.0141.16863
CCGAAGT2676650.0140.897231
AACATCG1976500.0140.823172
AGGTACA1587300.0140.099622
AACGTGA1330900.0140.030611
AAGGTAC1588200.0140.020251
GTATCAG1596600.0139.987053
Produced by FastQC (version 0.11.5)