FastQCFastQC Report
Mon 20 Mar 2023
SRR6211754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66562330
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5131600.7709465699292678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3787630.5690350683336957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2495670.37493729561450145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2175840.32688759543122964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1754500.2635875276601645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1119830.16823780056978174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1079960.16224792611676905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA947320.1423207390726857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG937640.14086646305800896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC812900.12212613350524237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC812330.122040499483717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG800230.12022265446537102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT702730.10557472973076512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT27171650.0136.246081
AGCAGTG28041200.0131.884452
GTGGTAT28043300.0131.873836
GGTATCA28032650.0131.72418
TGGTATC28033650.0131.634417
AGTGGTA28121800.0131.56925
CAGTGGT28124600.0131.514984
GTATCAA28200000.0131.20929
GCAGTGG29569300.0125.059833
GGTATAA267250.035.96288
TGGTATA281350.034.1605077
CAACGCA28007450.027.19864310-14
ATCAACG28060450.026.99989910-14
CGCAGAG28096550.026.97733515-19
AACGCAG28288100.026.94900710-14
TCAACGC28242200.026.9275510-14
ACATGGG27746200.026.92110420-24
TACATGG28125050.026.90178320-24
CAGAGTA28324800.026.87396615-19
GCAGAGT28268800.026.87005415-19
Produced by FastQC (version 0.11.5)