FastQCFastQC Report
Mon 20 Mar 2023
SRR6211753_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211753_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49278170
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC7925500.0143.862381
GCATACA7944950.0143.510212
ACGCTTA2326800.0142.074462
CGAAGTA3499200.0141.745322
CCGAAGT3503700.0141.563261
CGAACTT2703150.0141.480581
GAGCTGA2988100.0140.808731
AGCTGAA2991900.0140.62992
CATACAG2531900.0140.37823
GAACTTA2732000.0139.986542
CATACAT2627950.0138.648933
AACGCTT2387450.0138.465261
ACTTCGA873250.0138.144792
CTCAATG1324800.0137.797851
GATAGAC1443000.0137.573411
ACTAGTA1442450.0136.39452
ACGTATC697800.0135.466871
CGCTTAG775450.0135.270953
GCTGAAT1070200.0135.11723
GACTAGT1456800.0135.050961
Produced by FastQC (version 0.11.5)