FastQCFastQC Report
Mon 20 Mar 2023
SRR6211753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49278170
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2888730.5862088628697049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2281710.46302652878546424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT25230400.0137.74051
GTGGTAT25603650.0135.317986
TGGTATC25536900.0135.303487
AGTGGTA25622900.0135.245185
GGTATCA25557600.0135.244958
AGCAGTG25640000.0135.174132
CAGTGGT25694950.0134.833374
GTATCAA25714650.0134.76469
GCAGTGG26710900.0129.691713
TGGTATA293500.037.6193477
GGTATAA299700.036.889488
CGGTCTG77350.031.029755145
GTTGTAT314500.030.0360776
GCAGTTG318750.029.5976493
CAACGCA25513850.027.81187810-14
AACGCAG25636950.027.67844810-14
TCAACGC25651050.027.6629510-14
ATCAACG25565500.027.6626210-14
CGCAGAG25511250.027.6562415-19
AGAGTAC25599600.027.65617615-19
Produced by FastQC (version 0.11.5)