FastQCFastQC Report
Mon 20 Mar 2023
SRR6211752_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211752_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63470696
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA7020900.0144.000381
TGCCTAA7051000.0143.385652
CGCATAC5515150.0143.095871
GATAGAC5487300.0142.964691
GCATACA5535250.0142.576252
GATCGCA3418400.0142.575012
CGAACTT3932500.0142.419131
AGATCGC3436850.0141.809631
GCCTAAG2643000.0141.190953
GAACTTA3967650.0141.157422
ATAGACA5566400.0140.933122
GACTGGA2868950.0140.570572
GCCTAAT2204400.0140.342453
CGACTGG2874500.0140.299151
ACGTGAT2021150.0139.35772
GTCACTA1425050.0138.928652
AGTCACT1428600.0138.583421
ATCGCAT1217300.0138.185533
CATACAG1818650.0137.737673
TAGACAT1910550.0137.64273
Produced by FastQC (version 0.11.5)