FastQCFastQC Report
Mon 20 Mar 2023
SRR6211751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56274650
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA7054300.0144.041551
AGGTACA14854600.0143.870252
AAGGTAC14857350.0143.843631
TGCCTAA7084800.0143.421452
ACTTCGA3250550.0142.913352
GGTACAT5064400.0141.610153
CACTTCG3284850.0141.421071
GCCTAAG2749050.0141.141313
GGTACAG4825650.0141.119723
GCCTAAT2267450.0140.575563
GATAGAC2380100.0140.212051
ACGTGAT2121750.0139.82112
AACATCG2074000.0139.698522
GGTACAA4336900.0139.03823
AACGTGA2147950.0138.115621
CAAGCTA1490250.0137.473312
CTTCGAT1196100.0137.381453
GTACAGT1677050.0137.273534
ATAGACA2440200.0136.758762
CTCAATG1217000.0136.55311
Produced by FastQC (version 0.11.5)