FastQCFastQC Report
Mon 20 Mar 2023
SRR6211750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58411209
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1379650.23619610407310693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1139240.19503790787826358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAATCCCAGCACTTTGGGA972110.16642524896206137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG712670.12200911643516915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG712070.12190639642469991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT38538750.0142.036851
AGCAGTG39257800.0139.124632
AGTGGTA39287400.0138.926965
TGGTATC39265100.0138.756047
CAGTGGT39410600.0138.469644
GTGGTAT39416350.0138.450746
GGTATCA39508750.0137.961248
GTATCAA39693950.0137.591169
GCAGTGG40516800.0134.697373
GTTGTAT296350.037.1841556
GCAGTTG314750.035.3179053
TGGTATA332950.034.8603327
CAGTTGT337550.032.736644
TTGTATC340000.032.5809447
GGTATAA366800.031.5049088
AGTTGTA370400.029.9069125
CAACGCA39464950.028.42288410-14
AACGCAG39548500.028.35400210-14
TCAACGC39523050.028.35355210-14
ATCAACG39460600.028.2989210-14
Produced by FastQC (version 0.11.5)