FastQCFastQC Report
Mon 20 Mar 2023
SRR6211748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89752158
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24867032.770633102771746No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8029250.8946024451022114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6753010.7524064212472752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5726880.6380771368193732No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2443910.2722954026353327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC2747150.0141.326921
AGTTAGC2214500.0139.971922
GGCTAAC2264250.0138.852312
ATGAATC1743950.0138.835072
ATAGACA2819150.0138.821642
TAAGGTC2075000.0138.461072
CGAGTAA2125350.0138.348772
GCGAGTA2125600.0137.715761
TATCAGC1778700.0137.406041
GATGTAC2351950.0137.302812
AGAGATC2046100.0136.851722
AGGCTAA2297200.0136.63221
AGCACCT2674500.0136.630071
CCGACAA2577700.0136.460511
CAGATTC2164500.0136.45522
ATCAGCA1788000.0135.68452
TGGAACA2216500.0135.530431
TCACGCA2325050.0135.484572
TAGACAT1435200.0135.314243
GCTAACT1178250.0135.129273
Produced by FastQC (version 0.11.5)