FastQCFastQC Report
Mon 20 Mar 2023
SRR6211748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89752158
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1531997217.069196263782317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12033851.340786702866799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5844120.6511397753801085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5405890.6023130942433719No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3767470.4197637231185015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3111790.3467092122732024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3011330.33551616664192074No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2720870.30315371358536025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2425980.2702976790819893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC2187910.2437724115781149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2082480.2320256188157615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1855620.20674934634997855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1815580.20228817227993556No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1546430.17230003539302086No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1517990.16913130935525808No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1475700.16441944493412627No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1463170.16302337822339605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1354950.15096572942569247No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1153410.12851055904416248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1121730.12498083890083178No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1102750.12286612651698024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1054720.11751472315573738No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1052350.11725066265259047No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1041370.11602729373927699No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC913180.10174462880324282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT111754850.064.871291
AGTGGTA115748150.062.60115
AGCAGTG115815350.062.5532
GTATCAA116042500.062.4558879
CAGTGGT117289150.061.7726064
GGTATCA117324250.061.7140438
GCAGTGG118126800.061.310713
TGGTATC118696300.060.9981167
GTGGTAT118858050.060.968376
ATGGGGG4373100.022.71972725-29
TGACTGG452300.022.2710192
ACCTAGC884450.021.92254335-39
ATGGGGC3260350.021.8133325-29
GACTGGA464350.021.6000443
TAGCCCC883900.021.58266435-39
GGGGCTA673150.021.40019825-29
ATGGGGT2448600.021.37326425-29
GGGCTAA974000.021.3439125-29
GTGACTG474600.021.2397461
ATGGGAT4934350.020.59863725-29
Produced by FastQC (version 0.11.5)