FastQCFastQC Report
Mon 20 Mar 2023
SRR6211746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121008875
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17953501.483651509031879No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8281050.6843341035936413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5351420.4422336791413027No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5316740.43936777364470164No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1824510.15077489151105652No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1237200.10224043484413849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTC8764800.0142.081242
GATGTAC12963250.0142.078522
AGATGTA13043100.0141.575011
CTAAGGT8794250.0141.036071
ATGAATC3443300.0140.441932
GATAGAC2140550.0139.521181
TATCAGC2913300.0139.231961
TGGAACA4595400.0138.82351
AAGGTCA2832450.0137.929433
AGAGATC3158050.0137.848332
ATCAGCA2937750.0137.308382
AGCACCT3464050.0137.020941
AAGGTCG2370800.0136.941793
ATAGCGA973550.0136.695021
CCTAATC3030250.0136.665241
AGTTAGC1404550.0136.190172
GTGCGAA1963000.0136.04832
GGAACAA4664750.0135.957662
CTAATCC3056500.0135.769592
AAGGTCT3904750.0135.536123
Produced by FastQC (version 0.11.5)