FastQCFastQC Report
Mon 20 Mar 2023
SRR6211745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133346865
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15975611.1980491629855716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA5890340.44173066985864273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT4818700.3613658258857454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3780360.2834982284735378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3041630.22809910079250834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2428270.182101768946724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2338840.17539519957968266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2034610.15258026501035477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1947630.14605742699687765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1786730.13399115157300473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC1742000.13063674200364592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT88138450.0130.661931
AGTGGTA89683200.0128.213785
GTGGTAT89754750.0128.083426
AGCAGTG89914950.0128.025822
CAGTGGT90019200.0127.872864
GGTATCA89877050.0127.616518
TGGTATC89868000.0127.599647
GTATCAA90176800.0127.232059
GCAGTGG91173150.0126.2364653
GGTATGA755150.033.4769678
TGGTATG805000.031.4471827
GTATGAA944850.026.831879
CATGGGG35407250.026.59527225-29
CAACGCA89697500.026.26472910-14
ATGGGGG11874850.026.2352625-29
ATGGGGT9110050.026.19525725-29
ATCAACG89738600.026.1639310-14
AACGCAG90267800.026.15609610-14
CGCAGAG90011500.026.15433515-19
TCAACGC90182000.026.09927210-14
Produced by FastQC (version 0.11.5)