FastQCFastQC Report
Mon 20 Mar 2023
SRR6211743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184202754
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC16928850.0141.346372
AGATTCG5589550.0140.28943
ACAGATT17236450.0139.13821
ATGCCTA3737500.0139.02041
CGCATAC3141350.0138.254091
GATAGAC2582050.0138.166061
AGAGTCA4212450.0137.011831
ACTTCGA2823650.0136.674132
GATTCGA1662050.0136.438354
ACGTATC1656650.0136.247631
TAGCGAC1421450.0136.186982
GAGTCAA4256900.0136.085242
CCAGTTC7234200.0136.044891
AGTGGTC5020850.0135.942861
CAGTTCA7301600.0135.66832
AGATCGC2378350.0135.435041
ATAGCGA1422000.0135.305511
TGCCTAA3857150.0135.302632
GCGAGTA2187600.0135.208311
GATCGCA2401050.0134.78782
Produced by FastQC (version 0.11.5)