FastQCFastQC Report
Mon 20 Mar 2023
SRR6211743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184202754
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30270701.6433359079962506No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20156351.0942480262808667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8103440.4399195899101487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4750680.2579049388154099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4018950.21818077703659086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3962170.21509830412198944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3087580.1676185579722657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2892290.15701665350779717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2612160.14180895471302238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2211140.12003837901359499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1969820.1069375976865145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT105446700.0121.5614551
AGTGGTA106470900.0119.6072855
GTATCAA106519850.0119.41129
AGCAGTG106509750.0119.40532
GGTATCA105660500.0119.399468
GTGGTAT106847450.0119.1749956
CAGTGGT106932600.0118.9539264
TGGTATC106138900.0118.837767
GCAGTGG108630350.0116.995193
GGTATAA1651800.078.945558
TGGTATA1717350.075.919647
GCAGTTG1868700.055.260953
GTTGTAT1900250.054.5140156
TGTATCA1896850.054.4637268
GTATAAA2415700.054.1540159
TTGTATC1966800.052.5669447
CAGTTGT2128050.048.677474
AGCAGTT2190150.047.285092
AGTTGTA2393950.043.315995
CATGGGG35382250.026.87243825-29
Produced by FastQC (version 0.11.5)