FastQCFastQC Report
Mon 20 Mar 2023
SRR6211742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115877554
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5382160.46446959002948923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4987250.43038965078603575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3463040.29885339139968387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3203640.276467692785438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2197140.18960876581844316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2188030.18882259112925354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1849730.1596279811014996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1835330.15838529004504187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1789280.154411267604078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT1546430.13345380072485824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1515780.13080876733038393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG1443230.1245478481535777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1365310.11782350877030075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1210020.10442229389826437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1208720.10431010651122305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT56465950.0137.532471
AGTGGTA57042250.0135.851785
TGGTATC56892900.0135.747927
GTGGTAT57072450.0135.732596
AGCAGTG57186700.0135.474912
CAGTGGT57189800.0135.459634
GGTATCA57037150.0135.457618
GTATCAA57385950.0134.999119
GCAGTGG59908700.0129.264713
CGAGTAA136850.061.675352
TGGTATA599050.047.1472177
GGTATAA616100.045.9125758
GTTGTAT559300.037.9861456
TTGTATC591150.035.927357
CAGTTGT708350.030.2167554
TGTATCA707800.030.0266328
GTATAAA980300.028.9653479
GCAGTTG741600.028.7830093
CAACGCA57025900.027.60216510-14
ATCAACG57050550.027.51147810-14
Produced by FastQC (version 0.11.5)