FastQCFastQC Report
Mon 20 Mar 2023
SRR6211741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71734251
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA8847150.0141.742922
ACGTATC3305800.0141.688081
CGCATAC2335750.0141.212391
AGAGTCA3062250.0140.604171
CAAGCTA3068900.0140.307542
CGCTTAG3061600.0140.192583
GAGTCAA3064450.0139.888462
CGAACTT3621500.0139.686051
CGTGAGA2543950.0139.509612
AGTGGTC3617350.0139.29731
GCATACA2409500.0138.918432
CGCTTAT3169750.0138.72213
ACGTGAT1536700.0138.557742
ACAAGCT3120950.0138.524251
GTGGTCA3642700.0138.267462
CCGTGAG2526150.0138.213351
CGCTTAA2787750.0138.122673
AGTACAA3831900.0137.683321
GAACTTA3733550.0137.134952
AACGTGA1548800.0137.010931
Produced by FastQC (version 0.11.5)