FastQCFastQC Report
Mon 20 Mar 2023
SRR6211741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71734251
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6105760.8511638324626822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5775160.8050770614444696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2801660.39056098878065937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2765260.3854867042523383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT2459610.34287804859076315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2126290.2964121002671374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT2067960.288280698713924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2060000.2871710474819065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1446470.20164286652968608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1442530.20109361705052164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT1350740.1882977770270439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG1276470.1779442849413734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC1180470.16456155651503213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC1104960.15403520418718808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC960900.1339527473424097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC918160.12799464512426567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA904260.12605693757086833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC795050.11083268995169407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA739240.10305258501967213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT29006150.0134.970461
GTGGTAT29257500.0133.079136
GGTATCA29090650.0132.939568
TGGTATC29120400.0132.789667
CAGTGGT29304500.0132.754554
AGTGGTA29371000.0132.577455
GTATCAA29377750.0132.52519
AGCAGTG29515000.0131.791932
GCAGTGG30201450.0128.714923
GGTATAA386350.076.759168
TGGTATA405550.073.249417
TGTATCA473600.054.636868
GTATAAA565950.052.578329
GTTGTAT496400.052.272396
TTGTATC519200.049.9630477
CAGTTGT533150.048.6546364
AGCAGTT538800.048.1726952
GCAGTTG646300.040.0816353
CGAACTT67350.031.5870721
AGTTGTA923200.028.2931585
Produced by FastQC (version 0.11.5)