FastQCFastQC Report
Mon 20 Mar 2023
SRR6211740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81632636
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4186140.5128022571756717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA4003230.4903957774927175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2948630.3612072504923154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2379010.29142878590861626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2043670.2503496273230721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1600450.1960551659755297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1585680.19424584059738068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1297500.1589437832192507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG1099140.13464467814073772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1033370.12658785145686094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT853550.10455989685301845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT44481800.0136.675481
GTGGTAT44888700.0135.442326
GGTATCA44808050.0135.436378
TGGTATC44886450.0135.187477
GTATCAA44980650.0134.951079
AGCAGTG45128300.0134.830672
CAGTGGT45139000.0134.745334
AGTGGTA45156400.0134.715555
GCAGTGG46601100.0130.528993
CAACGCA44653500.027.69649710-14
TCAACGC44783100.027.58732410-14
CGCAGAG44815550.027.54477315-19
ATCAACG44748250.027.54364810-14
AGAGTAC45030950.027.45907415-19
ACATGGG44225650.027.45233320-24
AACGCAG45109200.027.44972410-14
TACATGG44880400.027.4469520-24
GTACATG44926950.027.44487820-24
GCAGAGT45100700.027.42155315-19
CAGAGTA45137950.027.40998615-19
Produced by FastQC (version 0.11.5)