FastQCFastQC Report
Mon 20 Mar 2023
SRR6211739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110015853
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3884950.0141.606551
CAGCAGA6280800.0141.268882
CGCATAC4349050.0141.146241
GCATACA4353600.0140.998732
ACAGCAG6304250.0140.74341
AGGTACA5778900.0140.443072
ATGCCTA3638900.0139.950581
AAGGTAC5799400.0139.946621
GTCACTA1815450.0139.545172
ATAGACA3949050.0139.308032
AACATCG4422850.0138.854552
ACGCTTA1983900.0138.451972
GACGGAT1155100.0138.431693
ACGTATC2082150.0138.339341
ACGCTCG2902300.0138.303241
ACTAGTA3464250.0138.207662
CTCAATG3825350.0137.898031
CGCTCGA2912750.0137.807052
TGCCTAA3696500.0137.769842
CATACCA7324450.0137.383441
Produced by FastQC (version 0.11.5)