FastQCFastQC Report
Mon 20 Mar 2023
SRR6211739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110015853
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7012770.6374326798156988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3293690.29938321707145243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA3044130.27669921352152765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2774830.25222092310641814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2000670.18185288260229188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1980560.18002496422038378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1636160.14872038487035136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1463250.13300355904162287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1101700.10014011344346892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT53447350.0133.34471
GTGGTAT53766500.0132.608846
GGTATCA53788900.0132.564338
TGGTATC53934150.0132.211987
GTATCAA54060400.0131.943569
AGCAGTG54137900.0131.659522
CAGTGGT54185800.0131.578814
AGTGGTA54292300.0131.315165
GCAGTGG55168750.0129.214143
CAACGCA53535700.027.11645310-14
CATGGGG19536450.027.01007325-29
ACATGGG52702100.026.96621520-24
ATCAACG53735450.026.94562110-14
CGCAGAG53657850.026.9387615-19
TCAACGC53883600.026.93806510-14
TACATGG53776750.026.88322820-24
AGAGTAC53931050.026.84229715-19
GTACATG53928850.026.8281720-24
GCAGAGT54001950.026.81614515-19
CAGAGTA54054550.026.79875615-19
Produced by FastQC (version 0.11.5)