FastQCFastQC Report
Mon 20 Mar 2023
SRR6211737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44623488
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3220150.7216266913066052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2429530.5444509402761165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1232300.2761550150449915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1118220.2505900031839734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1074920.24088659317711786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA830400.18609033879198328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC591170.13247955874717818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT572530.12830238640242556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA551670.12362771820974641No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT489190.10962612335458852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGCCAACTCCAGGGATTC458620.10277547107030272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT22799050.0133.732511
GTGGTAT22972700.0132.777926
GGTATCA22976700.0132.76668
TGGTATC23009300.0132.58337
GTATCAA23099250.0132.098989
CAGTGGT23108500.0131.982744
AGCAGTG23111400.0131.937352
AGTGGTA23178400.0131.568515
GCAGTGG23505150.0129.74283
CAACGCA22866150.027.1619610-14
TCAACGC22965650.027.03857810-14
CATGGGG8284850.027.0191325-29
ATCAACG22944750.027.00086410-14
ACATGGG22537300.026.98735620-24
CGCAGAG22927850.026.97652215-19
TACATGG22952600.026.93870420-24
GTACATG22985600.026.91787320-24
GCAGAGT23079050.026.8448115-19
CAGAGTA23105800.026.82201615-19
AGTACAT23034150.026.8110920-24
Produced by FastQC (version 0.11.5)