FastQCFastQC Report
Mon 20 Mar 2023
SRR6211736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86719651
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4056140.4677302033883877No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2280930.2630234293724268No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2120860.2445650986302978No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1631210.18810154113742916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA3181800.0138.953431
CTCAATG3896200.0138.597981
GAGTCAA3193350.0138.14822
ACGTATC1799400.0138.075411
ATGCCTA2887600.0138.068271
AGATCGC3849050.0137.998631
GATCGCA3849600.0137.645972
GAGCTGA2857900.0137.34871
ACTTCGA1573600.0137.226492
ACGTGAT1578100.0137.198012
TGCCTAA2924700.0136.112372
AGCTGAA2873100.0135.804062
GACTAGT1636150.0135.618071
GTCACTA1488450.0135.29572
AACGTGA1585050.0135.089461
CTAAGTG3418850.0134.047583
ACTAGTA1649500.0133.593832
TAAGTGC1306750.0133.564474
CTAAGTC1793900.0132.985093
TCAATGA4034500.0132.804892
Produced by FastQC (version 0.11.5)