FastQCFastQC Report
Mon 20 Mar 2023
SRR6211735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62564680
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3645990.5827553181763256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1968240.31459283416777645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1568120.2506398178652876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT34204650.0135.748641
TGGTATC34207150.0134.62397
AGTGGTA34421250.0134.602895
GGTATCA34223000.0134.371648
GTGGTAT34517400.0134.340976
AGCAGTG34511000.0134.309162
CAGTGGT34557350.0134.009584
GTATCAA34535950.0133.99529
GCAGTGG35492950.0130.613343
GTTGTAT461650.064.407416
TGGTATA486400.063.1722157
GGTATAA488350.062.6546678
TTGTATC485850.061.125237
GCAGTTG493700.060.10983
CAGTTGT500350.059.384414
GTATCGA75450.055.2351579
GGTATCG78650.052.712948
AGTTGTA590100.050.5217065
TGTATCA601050.049.3737648
AGCAGTT669650.044.498732
Produced by FastQC (version 0.11.5)