FastQCFastQC Report
Mon 20 Mar 2023
SRR6211734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62599902
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1045910.1670785363210313No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1010160.16136766476088094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3443800.0142.219041
GTCGTAG3065600.0141.40711
TCGTAGA3036550.0141.149142
TATCAGC1722150.0140.353411
ATAGACA3499100.0139.623632
GCGAGTA1568700.0139.538931
ATCCGTC1880950.0138.822
TCACGCA2202150.0138.718372
ATCAGCA1727200.0138.150772
ATGAATC1744750.0138.04082
AGTTAGC1244200.0137.212252
TAGACAT1271450.0137.046433
GTGCGAA1169100.0137.013722
ATCATTC2454450.0137.002431
CCTAATC2065750.0136.649191
ATAGCGA553150.0136.554571
CGAGTAA1589000.0136.53242
GCCACAT2371950.0136.433691
TTCACGC2277250.0136.028991
TAGCGAC556950.0135.831312
Produced by FastQC (version 0.11.5)