FastQCFastQC Report
Mon 20 Mar 2023
SRR6211734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62599902
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25831674.126471316201101No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4514930.7212359533725787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3968760.6339882129527934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1891410.30214264552682524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1271790.20316165990164012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1181990.1888165895211785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC892350.14254814648112388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT841720.13446027439467878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC826810.13207848152861326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT811930.1297014810023185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT770130.12302415425506576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG693280.11074777720898031No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA668940.10685959220830729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTCTTAGCGGTGGATCA645970.1031902573904988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT32169700.090.402181
GTATCAA32548950.089.016139
AGTGGTA32807650.088.881915
AGCAGTG32855600.088.391092
GGTATCA32874500.088.338968
CAGTGGT33287800.087.558364
GTGGTAT33199050.087.516546
TGGTATC33146600.087.333777
GCAGTGG33789900.086.2405853
GGTATAA349300.034.8351368
TGGTATA375400.032.4894187
AGCGGTA110450.026.5960545
CGGTATC118150.025.0455677
CATGGGG7337300.024.94130725-29
GCGGTAT120400.024.3981256
ATGGGGG2969250.023.91745825-29
CGTCGAT118650.022.427860-64
ATGGGGC1906950.022.16162125-29
ATGGGAG3532250.021.21860525-29
ATGGGGT1289150.021.18569425-29
Produced by FastQC (version 0.11.5)