FastQCFastQC Report
Mon 20 Mar 2023
SRR6211733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96024969
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5239510.5456403740156376No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3100280.32286185898169883No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2448990.2550367915245044No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1518570.15814324293090895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT12003550.0141.422361
AGATCGC4811250.0140.34711
AGTCACT3469150.0140.219131
ACGCTTA3521700.0140.198912
GATCGCA4770000.0139.920732
GTCACTA3448550.0139.880052
GACTAGT2984000.0139.318731
GAACTTA12104400.0139.299932
ATGCCTA2296350.0139.067611
TGCCTAA2300450.0137.20852
TGAGCCA3159050.0136.814242
CTCAATG2636900.0136.678021
CGTGAGA1966550.0136.408832
ACTAGTA3044300.0135.930422
AACTTAG4178200.0135.842383
GACGGAT1203250.0135.823473
CTGAGCC3208050.0135.736511
CAAGCTA2429700.0135.21432
ACGTATC1143550.0135.138871
AGTGGTC2168750.0135.013821
Produced by FastQC (version 0.11.5)